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Gene Review:

gltP - glutamate/aspartate:proton symporter

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4070, JW4038

Wallace, B. et al., Peekhaus, N. et al., Jacobs, M.H. et al., Tolner, B. et al.

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Disease relevance of gltP

The gene (designated gltP) is probably identical to a gene recently cloned from E. coli B (Y. Deguchi, I. Yamato, and Y. Anraku, J. Bacteriol. 171:1314-1319) [1].
A 1.6-kilobase DNA fragment containing gltP was subcloned into the expression plasmids pT7-5 and pT7-6, and its product was identified by a phage T7 RNA polymerase-T7 promoter coupled system (S. Tabor and C. C. Richardson, Proc. Natl. Acad. Sci. USA 82:1074-1078) as a polypeptide with an apparent mass of 38 kilodaltons [1].

High impact information on gltP

Both glutamate uptake and chemotaxis were recovered when the gltP gene, coding for the H(+)-linked glutamate carrier of E. coli, was expressed in R. sphaeroides MJ7 [2].
Although overall glutamate uptake in E. coli was increased, the protein encoded by the cloned fragment repressed the secondary H+/glutamate transport system GltP by interaction with the promoter region of the gltP gene [3].
When expressed in E. coli, Grp repressed the secondary H+/glutamate transport system GltP by binding to the regulatory regions of the gltP gene [3].
Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12 [4].
A portion of the gltP polypeptide was associated with the cytoplasmic membrane [1].

References

Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12. Wallace, B., Yang, Y.J., Hong, J.S., Lum, D. J. Bacteriol. (1990) [Pubmed]
Expression of the gltP gene of Escherichia coli in a glutamate transport-deficient mutant of Rhodobacter sphaeroides restores chemotaxis to glutamate. Jacobs, M.H., van der Heide, T., Tolner, B., Driessen, A.J., Konings, W.N. Mol. Microbiol. (1995) [Pubmed]
The glutamate uptake regulatory protein (Grp) of Zymomonas mobilis and its relation to the global regulator Lrp of Escherichia coli. Peekhaus, N., Tolner, B., Poolman, B., Krämer, R. J. Bacteriol. (1995) [Pubmed]
Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12. Tolner, B., Poolman, B., Wallace, B., Konings, W.N. J. Bacteriol. (1992) [Pubmed]

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