Disease relevance of dnaT

• Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication [1].

High impact information on dnaT

• (ii) An extract from a dnaT strain does not support replication of the plasmid pBR322 in vitro; addition of purified protein i restores its activity [1].
• The P-14 gene is transcribed from an upstream promoter, and its transcript extends through dnaT and dnaC [2].
• These results indicate that dnaT and dnaC constitute an operon with two unknown genes whose products may be associated with a membrane [2].
• Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome [2].
• Early replicative intermediates with newly synthesized leading strand, approximately 1 kilobase pair long, immediately downstream of the replication origin accumulate in products synthesized in extracts from a dnaT strain that lacks primosomal protein i or in wild-type extracts supplemented with anti-protein i antibody [3].

Biological context of dnaT

• (i) A multicopy plasmid carrying only the protein i gene suppresses the temperature-sensitive phenotype of a dnaT strain and restores the ability of the strain to carry out stable DNA replication in the absence of protein synthesis [1].
• These results indicate that protein i is encoded by dnaT and that it is essential for chromosomal DNA replication and is involved in the induction of stable DNA replication during the SOS response [1].
• An upstream open reading frame, separated by just 2 base pairs from the coding region of dnaC, encodes the COOH-terminal half of the dnaT product (protein i; Masai, H., Bond, M. W., and Arai, K. (1986) Proc. Natl. Acad. Sci. U. S. A. 83, 1256-1260) [4].

References