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Gene Review:

psbE - cytochrome B559 subunit alpha

Synechocystis sp. PCC 6803

This record was discontinued.

Pakrasi, H.B. et al., Shukla, V.K. et al., Pakrasi, H.B. et al.

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Disease relevance of psbE

The genes encoding the two subunits (alpha and beta) of the cytochrome b559 (cyt b559) protein, psbE and psbF, were cloned from the unicellular, transformable cyanobacterium, Synechocystis 6803 [1].

High impact information on psbE

Northern blot analysis showed that these mutations did not affect the stability of the polycistronic mRNA that encompasses both the psbE and the psbF genes, encoding the alpha and the beta subunits, respectively [2].
Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II [1].
Cartridge mutagenesis techniques were used to generate a deletion mutant of Synechocystis 6803 in which the psbE and psbF genes were replaced by a kanamycin-resistance gene cartridge [1].
A high degree of homology was found between the cyanobacterial and green plant chloroplastidic psbE and psbE genes and in the amino acid sequences of their corresponding protein products [1].
Nucleotide sequence analysis showed that the primary lesion in CP6t16 is an ochre mutation introducing a translational stop codon in the psbE gene, encoding the alpha-subunit of cytochrome b559, an integral component of the PSII complex [3].

References

Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II. Pakrasi, H.B., Williams, J.G., Arntzen, C.J. EMBO J. (1988) [Pubmed]
Site directed mutagenesis of the heme axial ligands of cytochrome b559 affects the stability of the photosystem II complex. Pakrasi, H.B., De Ciechi, P., Whitmarsh, J. EMBO J. (1991) [Pubmed]
The D1 protein of the photosystem II reaction-centre complex accumulates in the absence of D2: analysis of a mutant of the cyanobacterium Synechocystis sp. PCC 6803 lacking cytochrome b559. Shukla, V.K., Stanbekova, G.E., Shestakov, S.V., Pakrasi, H.B. Mol. Microbiol. (1992) [Pubmed]

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