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Gene Review:

psbF - cytochrome B559 subunit beta

Synechocystis sp. PCC 6803

This record was discontinued.

Pakrasi, H.B. et al., Pakrasi, H.B. et al., Pakrasi, H.B. et al.

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Disease relevance of psbF

The genes encoding the two subunits (alpha and beta) of the cytochrome b559 (cyt b559) protein, psbE and psbF, were cloned from the unicellular, transformable cyanobacterium, Synechocystis 6803 [1].

High impact information on psbF

Northern blot analysis showed that these mutations did not affect the stability of the polycistronic mRNA that encompasses both the psbE and the psbF genes, encoding the alpha and the beta subunits, respectively [2].
Oligonucleotide-directed mutagenesis techniques were used to delete the psbF gene, encoding the beta subunit of the cytochrome b559 protein of the photosystem II complex in the cyanobacterium, Synechocystis 6803 [3].
The newly generated mutant, T256, had only one gene, psbF, deleted from the genome [3].

Chemical compound and disease context of psbF

Cartridge mutagenesis techniques were used to generate a deletion mutant of Synechocystis 6803 in which the psbE and psbF genes were replaced by a kanamycin-resistance gene cartridge [1].

References

Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II. Pakrasi, H.B., Williams, J.G., Arntzen, C.J. EMBO J. (1988) [Pubmed]
Site directed mutagenesis of the heme axial ligands of cytochrome b559 affects the stability of the photosystem II complex. Pakrasi, H.B., De Ciechi, P., Whitmarsh, J. EMBO J. (1991) [Pubmed]
Targeted deletion mutagenesis of the beta subunit of cytochrome b559 protein destabilizes the reaction center of photosystem II. Pakrasi, H.B., Nyhus, K.J., Granok, H. Z. Naturforsch., C, J. Biosci. (1990) [Pubmed]

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