Disease relevance of groEL

• Using a groEL gene of Synechococcus sp. PCC 7942 as a DNA probe, a 4.8-kilobase pair (kbp) BamHI fragment of chromosomal DNA of Synechocystis sp. PCC 6803 was cloned [1].

High impact information on groEL

• The amino acid sequences deduced from the nucleotide sequences of the two open reading frames are identical to those gained from N-terminal sequencing of purified groEL and groES proteins [1].
• A second groEL-like gene, organized in a groESL operon is present in the genome of Synechocystis sp. PCC 6803 [1].
• Southern blot analysis indicates that only one groESL operon is present in the genomic DNA of Synechocystis sp. PCC 6803, whereas the existence of at least two copies of groEL-analogous genes are anticipated [1].

Anatomical context of groEL

• The coexistence of divergent groEL/cpn60 genes in different genomes in one cell offers insights into gene transfer from evolving chloroplasts to cell nuclei and convergent gene evolution in chlorophyll a/b versus chlorophyll a/c/phycobilin eukaryotic lineages [2].

References