Reduced phagocytosis of colloidal carriers using soluble CD47.
PURPOSE: This study was designed to illustrate the feasibility of using soluble CD47 protein to antagonize phagocytosis of colloidal drug carriers by macrophages. METHODS: Expression of CD47-streptavidin (CD47- SA) fusion protein was achieved in B21CodonPlus host cells following IPTG induction. Murine macrophage cell line J774A.1, expressing high levels of SIRPalpha, was selected as the biologic model system for phagocytosis. FITC-labeled perfluorocarbon (PFC) emulsions were used as the colloidal carriers to trigger phagocytosis. Microscopy (inverted light and UV-fluorescence) and flow cytometry were used to qualitatively and quantitatively determine the degree of phagocytosis, respectively. RESULTS: The bacterially expressed, purified CD47- SA had neither cytotoxic nor cytostatic effects when incubated with J774A.1 cells up to a concentration of 400 nM for 24 h. Phagocytosis of FITC-labeled PFC emulsions was significantly diminished when macrophages were pretreated with 100 nM CD47- SA for 1 h. CONCLUSIONS: We demonstrated that soluble CD47- SA antagonized phagocytosis of colloidal carriers to a significant degree by interaction with macrophage SIRPalpha.[1]References
- Reduced phagocytosis of colloidal carriers using soluble CD47. Hsu, Y.C., Acuña, M., Tahara, S.M., Peng, C.A. Pharm. Res. (2003) [Pubmed]
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