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Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3.

PRL-3 is a newly identified nonclassical protein tyrosine phosphatase associated with colorectal cancer metastasis. To prepare specific monoclonal antibody (MAb) against PRL-3, cDNAs of PRL-1, -2, and -3 were cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against PRL-3 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 3B6 had specific binding ability with PRL-3 protein in eukaryotic cells. This MAb will be a helpful tool for the detection of PRL-3 protein in the tissues and serum of colorectal cancer and other cancer patients.[1]

References

  1. Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3. Peng, L., Li, Y., Meng, L., Shou, C. Hybrid. Hybridomics (2004) [Pubmed]
 
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