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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes.

OBJECTIVE: Histamine stimulates nerve growth factor (NGF) secretion from cultured astrocytes. Histamine H(1)-receptor antagonists completely block its effect. In the present study, we determined the involvement of histamine-receptor subtypes in this process. MATERIALS AND METHODS: Radioligand-binding assay was used to establish the presence of histamine H(1)- and H(2)-receptors on new-born rat cortical astrocytes in primary culture. Histamine H(1)-, H(2)- and H(3)/H(4)-receptor ligands, and highly selective protein kinase C ( PKC) inhibitor were used to influence NGF secretion from cultured astrocytes. NGF, released into the culture medium, was measured by NGF-ELISA. RESULTS: Histamine H(1)-receptor agonists (histamine, selected histaprodifens) increased the secretion of NGF from cultured astrocytes in a concentration-dependent manner. H(1)-receptor antagonists/inverse agonists (mepyramine, triprolidine) and PKC inhibitor completely blocked the effect of histamine. Histamine H(2)- and H(3)-receptor agonists did not enhance NGF secretion significantly. In addition, H(2)- and H(3)/H(4)-receptor antagonists did not diminish histamine-stimulated NGF release. CONCLUSIONS: Our results indicate that histamine H(1)-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.[1]

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