Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Ichimura-Ohshima, Y. et al.

cDNA cloning and chromosome assignment of the gene for human brain 14-3-3 protein eta chain.

We present the nucleotide sequence of a cDNA clone of mRNA encoding human 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases and an endogenous inhibitor of protein kinase C. The 1,730-nucleotide sequence of the cloned cDNA contains 191 bp of a 5'-noncoding region, the complete 738 bp of coding region, and 801 bp of a 3'-noncoding region containing three canonical polyadenylation signals. The 14-3-3 protein eta chain cDNA encoded a polypeptide of 246 amino acids with a predicted molecular weight 28,196. The predicted amino acid sequence of human 14-3-3 protein eta was highly homologous to that of previously reported bovine and rat 14-3-3 proteins with only two amino acid differences. The sequence carries structural features as putative regions responsible for activation of tyrosine and tryptophan hydroxylases and for inhibition of Ca2+/phospholipid-dependent protein kinase C. Northern blot analysis demonstrated widespread expression of the 14-3-3 protein eta chain in cultured cell lines derived from various human tumors. These findings suggest the conservative functions of the 14-3-3 protein among species. Spot blot hybridization analysis with flow-sorted chromosomes showed that the human 14-3-3 protein eta chain gene is assigned to chromosome 22.[1]

References

cDNA cloning and chromosome assignment of the gene for human brain 14-3-3 protein eta chain. Ichimura-Ohshima, Y., Morii, K., Ichimura, T., Araki, K., Takahashi, Y., Isobe, T., Minoshima, S., Fukuyama, R., Shimizu, N., Kuwano, R. J. Neurosci. Res. (1992) [Pubmed]

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