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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Agrobacterium-mediated transformation of Sclerotinia sclerotiorum.

Ascospores from the phytopathogenic fungus Sclerotinia sclerotiorum were transformed to hygromycin B resistance by co-cultivation with Agrobacterium tumefaciens. Transformed spores germinated and grew on PDA supplemented with 100 ug/ml hygromycin B. The presence of mitotically stable hph gene integration at random sites in the genome was confirmed by PCR and Southern blot analysis. A transformation frequency of 8 x 10(-5) was achieved in five separate experiments. This study is the first report of success co-cultivating A. tumefaciens with S. sclerotiorum. This report of a reproducible Agrobacterium-mediated transformation method should allow the development of T-DNA tagging as a system for insertional mutagenesis in S. sclerotiorum and provide a simple and reliable method for genetic manipulation.[1]

References

  1. Agrobacterium-mediated transformation of Sclerotinia sclerotiorum. Weld, R.J., Eady, C.C., Ridgway, H.J. J. Microbiol. Methods (2006) [Pubmed]
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