Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts.
1-cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine residue, reduces a broad spectrum of hydroperoxides. This study was undertaken to examine changes in 1-cysPrx expression in human cataract samples, human lens epithelial (HLE B3) cell line, and rat organ-cultured lenses in response to oxidative insult induced by H(2)O(2) or transforming growth factor-beta1 ( TGF-beta1). Expression of 1-cysPrx mRNA and protein in HLE B3 cells increased in response to 2-8ngml(-1) TGF-beta1 and 50-75mum H(2)O(2) and then decreased below the control level at high doses (10ngml(-1) TGF-beta1 and 100-150mum H(2)O(2)), as determined by Northern blot and immunoblot analysis. This reduction coincided with the decrease of cell viability. Immunoreactive 1-cysPrx protein was measured in capsulorrhexis specimens obtained from patients with anterior subcapsular cataract (ASC), nuclear sclerosis (NS), cortical spokes (CS), posterior subcapsular cataract (PSC), or white mature cataract (WC) at the time of cataract surgery. Significant reduction of 1-cysPrx protein was observed in ASC, PSC, and WC samples, but there was no statistical difference in CS and NS samples relative to normal control. Also, rat lens explants were cultured with 10ngml(-1) TGF-beta1 for approximately 5 days or 500mum H(2)O(2) for approximately 2 days. Subsequently, expression of 1-cysPrx mRNA and protein in the lens capsules was evaluated. Rat lens explants treated with TGF-beta1 or H(2)O(2) developed a cataract similar to human ASC or WC, respectively, which resulted in a markedly decreased expression of 1-cysPrx mRNA and protein. Collectively, these findings show that expression patterns of 1-cysPrx gene in the lens are changed in response to oxidative stress, a major factor in the etiology of cataract.[1]References
- Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts. Pak, J.H., Kim, T.I., Joon Kim, M., Yong Kim, J., Choi, H.J., Kim, S.A., Tchah, H. Exp. Eye Res. (2006) [Pubmed]
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