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Chen, P. et al.

Chen, Cai, Yang, Zhou, Zhang, Domann, Fang,

Involvement of PKC, p38 MAPK and AP-2 in IL-1beta- induced expression of cyclooxygenase-2 in human pulmonary epithelial cells.

OBJECTIVE: The aim of this study was to identify the signal molecules involved in IL-1beta-induced expression of cyclooxygenase (COX)-2 in human pulmonary epithelial (A549) cells. METHODS: A549 cells were stimulated with IL-1beta in the presence or absence of H-7 (a protein kinase C inhibitor), SB203580 (a p38 mitogen-activated protein kinase inhibitor) and PD098059 (a mitogen-activated and extracellular regulated kinase kinase (MEK1) inhibitor). The A549 cells were also transfected with adenovirus vector encoding activator protein (AP)-2alpha, or a plasmid containing a dominant-negative gene (AP-2Delta), in the presence or absence of IL-1beta. RESULTS: IL-1beta induced expression of the COX-2 mRNA and protein in A549 cells in a time- and dose-dependent manner. SB203580 and H-7, but not PD098059, inhibited IL-1beta-induced expression of COX-2 protein. Overexpression of AP-2alpha increased expression of the COX-2 protein, whereas AP-2Delta decreased IL-1beta-induced COX-2 expression. CONCLUSION: Protein kinase C, p38 mitogen- activated protein kinase and transcriptional factor AP-2alpha may play important roles in regulating IL-1beta- induced COX-2 expression in human pulmonary epithelial cells.[1]

References

Involvement of PKC, p38 MAPK and AP-2 in IL-1beta-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells. Chen, P., Cai, Y., Yang, Z.G., Zhou, R., Zhang, G.S., Domann, F., Fang, X. Respirology (2006) [Pubmed]

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