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Choi, Y.G. et al.

Differential regulation of melanosomal proteins after hinokitiol treatment.

BACKGROUND: Melanogenesis is regulated by a series of enzymes under the control of microphthalmia-associated transcription factor (MITF). OBJECTIVE: The aim of this study was to examine melanosome-associated protein levels in Mel-Ab cells after hinokitiol treatment. METHODS: We measured melanin contents and analyzed melanosome-associated protein levels using Western blot and RT-PCR analysis. RESULTS: Hinokitiol markedly inhibited melanin synthesis and also reduced the protein levels of tyrosinase (TYR), tyrosinase-related protein 1 (TYRP-1), tyrosinase-related protein 2 (TYRP-2) and MITF in Mel-Ab cells. In addition, hinokitiol significantly increased the phosphorylations of extracellular signal-regulated kinases 1 and 2 (ERK1/2). Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that TYR and MITF mRNA levels were significantly decreased but that levels of TYRP-1 and TYRP-2 mRNA were unaffected by hinokitiol treatment. These results suggest that hinokitiol-induced ERK phosphorylation reduces MITF and TYR transcription, and mediates the action of hinokitiol on melanogenesis. Interestingly, the mRNAs of TYRP-1 and TYRP-2 were unaffected, although the protein levels of TYRP-1 and TYRP-2 were down-regulated. Thus, the effects of hinokitiol on the transcription of TYR may differ from its effects on TYRP-1 and TYRP-2. CONCLUSION: Therefore, we suggest that TYRP-1 and TYRP-2 may be regulated by post-translational degradation after hinokitiol treatment.[1]

References

Differential regulation of melanosomal proteins after hinokitiol treatment. Choi, Y.G., Bae, E.J., Kim, D.S., Park, S.H., Kwon, S.B., Na, J.I., Park, K.C. J. Dermatol. Sci. (2006) [Pubmed]

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