In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity.
PURPOSE: The metallopanstimulin-1 ( MPS-1) gene is a growth factor-inducible gene, which is highly expressed in many human cancers and may be involved in the progression towards tumor malignancy. However, it is unclear whether MPS-1 plays any role in gastric cancer development or progression. Our studies were designed to clarify the MPS-1 expression pattern and to explore its potential role in gastric cancer. EXPERIMENTAL DESIGN: The expression pattern of MPS-1 was determined in primary gastric cancer specimens and gastric cancer cell lines via immunohistochemistry and Western blotting. To investigate the functional significance of MPS-1 expression, three small interfering RNA (siRNA) expression plasmids were constructed and transfected into gastric cancer cell line SGC7901. The stable cell lines transfected with the siRNA targeting MPS-1 mRNA plasmids were selected and the biological features of these cells were examined. RESULTS: MPS-1 was overexpressed in 86% of the gastric cancer tissues and all gastric cancer cells. In addition, MPS-1 expression was significantly increased and corresponded with the tumor-node-metastasis clinical stage, and was significantly higher in the late stage (P < 0.01). The MPS-1 expression level was significantly decreased in the transfected cells with MPS-1-specific siRNA expression plasmid pRNAT-133. Furthermore, the stable transfected cancer cells exhibited an increase in the incidence of spontaneous apoptosis and a decrease in growth ability and tumorigenicity in nude mice. CONCLUSIONS: These results provide strong evidence that MPS-1 plays an important role in gastric cancer cell proliferation and development, and suggests that MPS-1 is a promising target for gastric cancer treatment.[1]References
- In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity. Wang, Y.W., Qu, Y., Li, J.F., Chen, X.H., Liu, B.Y., Gu, Q.L., Zhu, Z.G. Clin. Cancer Res. (2006) [Pubmed]
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