Cryopreservation of spermatozoa of black marlin, Makaira indica (Teleostei: istiophoridae).
As a first step towards the development of a method for the cryopreservation of black marlin spermatozoa, this study investigated the effect of dimethylsulfoxide (DMSO) concentration and pellet size on post-thaw spermatozoal motility. Spermatozoa were recovered from the spermatic duct of testes retrieved post-mortem from four adult black marlin caught in the Coral Sea spawning grounds of Australia. Undiluted spermatozoa were stored on ice for 4 to 10 hours during transport to shore, then evaluated for motility after activation in seawater (a volume ratio of 1 to 10). Spermatozoa were prepared for cryopreservation in pellets by extension (a volume ratio of 1 to 3) in a defined fish Ringer's solution to give two final DMSO concentrations of 2.5 percent or 5.0 percent. Diluted spermatozoa were frozen directly on a dry ice block in pellet sizes of either 0.25 ml or 0.50 ml. Frozen pellets were thawed in a water bath at 40 degree C for 60 seconds and assessed for post-thaw motility following activation in seawater. Spermatozoa recovered within 50 minutes of death and chilled on ice for 4 to 10 hours showed a mean (+/- SEM) motility immediately following activation of 91.6 +/- 7.9 percent. Fifty percent of the spermatozoa remained motile for approximately 4 to 5 minutes. Following cryopreservation, mean motility declined significantly across all cryoprotectant and pellet size combinations (P less than 0.001) but spermatozoa frozen in 2.5 percent DMSO showed higher motility than those frozen in 5.0 percet DMSO (P = 0.014). Pellet size had no effect on post-thaw motility (P = 0.179).[1]References
- Cryopreservation of spermatozoa of black marlin, Makaira indica (Teleostei: istiophoridae). van der Straten, K.M., Leung, L.K., Rossini, R., Johnston, S.D. Cryo letters. (2006) [Pubmed]
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