The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Binding Behavior of Subphthalocyanine-Tagged Testosterone with Human Serum Albumin at the n-Hexane/Water Interface.

Subphthalocyaninatoboron(III) was applied for the first time as a novel marker-tag of testosterone (Subpc-test) for the binding analysis with human serum albumin (HSA) at a liquid/liquid interface. The binding interaction of Subpc-test with HSA at the n-hexane/water interface was studied by UV-visible absorption and circular dichroism (CD) spectroscopy combined with a centrifugal liquid membrane cell at different pHs of aqueous solutions. Complementary studies by a high-speed stirring experiment and an interfacial tension measurement were also performed to characterize the interfacial adsorptivity of Subpc-test and HSA molecules, respectively. The n-hexane solution of Subpc-test showed no optical chirality, but the contact with the aqueous solution of HSA induced its optical chirality, clearly suggesting the formation of Subpc-test/HSA complexes at the n-hexane/water interface. Furthermore, pH profiles of CD signals showed that the interaction between Subpc-test and HSA was very sensitive to the neutral-to-base transition (N-B transition). Interfacial formation of the Subpc-test/HSA complex was studied in the presence of the site-selective ligand, furosemide (site I) or cefaclor (site II). The experimental results suggested that Subpc-test is bound to site I of the HSA molecule at the n-hexane/water interface. This technique using subphthalocyanine as a tag molecule and the liquid/liquid interface as a two-dimensional nanoreaction field will be useful for the evaluation of the interaction between drugs or hormones and proteins.[1]

References

 
WikiGenes - Universities