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Støttrup, N.B. et al.

Støttrup, Kristiansen, L??fgren, Hansen, Kimose, B??tker, Nielsen,

l-GLUTAMATE AND GLUTAMINE IMPROVE HAEMODYNAMIC FUNCTION AND RESTORE MYOCARDIAL GLYCOGEN CONTENT DURING POSTISCHAEMIC REPERFUSION: A RADIOACTIVE TRACER STUDY IN THE RAT ISOLATED HEART.

1. l-Glutamate and glutamine have been suggested to have cardioprotective effects. However, the issue is controversial and the metabolic mechanisms underlying a beneficial effect are not well understood. 2. In the present study we investigated the effects of l-glutamate and glutamine on haemodynamic recovery, the rate of de novo glycogen synthesis and myocardial glucose uptake during postischaemic reperfusion. 3. Hearts from male Wistar rats (250-300 g) were divided into three groups as follows: (i) control (n = 12); (ii) l-glutamate (n = 12); and (iii) glutamine (n = 12). Hearts were mounted in a Langendorff preparation and perfused with oxygenated Krebs'-Henseleit solution at 80 mmHg and 37C. Global ischaemia for 20 min was followed by 15 min reperfusion, during which l-glutamate (50 mmol/L) or glutamine (20 mmol/L) were administered. Left ventricular developed pressure (LVDP), de novo synthesis of glycogen using [(14)C]-glucose and myocardial glucose uptake using d-[2-(3)H]-glucose were measured. 4. l-Glutamate and glutamine increased postischaemic LVDP (P < 0.01 vs control hearts for both). l-Glutamate and glutamine increased de novo glycogen synthesis by 78% (P < 0.001) and 55% (P < 0.01), respectively. At the end of reperfusion, total myocardial glycogen content was increased by both l-glutamate and glutamine (5.7 +/- 0.3 and 6.2 +/- 0.7 mmol/g wet weight, respectively; P < 0.05 and 0.01, respectively) compared with that in control hearts (3.6 +/- 0.4 mmol/g wet weight). Neither l-glutamate nor glutamine affected myocardial glucose uptake during reperfusion. 5. Improved postischaemic haemodynamic recovery after l-glutamate and glutamine supplementation during reperfusion is associated with increased de novo glycogen synthesis, suggesting a favourable modulation of intracellular myocardial carbohydrate metabolism.[1]

References

l-GLUTAMATE AND GLUTAMINE IMPROVE HAEMODYNAMIC FUNCTION AND RESTORE MYOCARDIAL GLYCOGEN CONTENT DURING POSTISCHAEMIC REPERFUSION: A RADIOACTIVE TRACER STUDY IN THE RAT ISOLATED HEART. St??ttrup, N.B., Kristiansen, S.B., L??fgren, B., Hansen, B.F., Kimose, H.H., B??tker, H.E., Nielsen, T.T. Clin. Exp. Pharmacol. Physiol. (2006) [Pubmed]

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