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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Improved production of ethanol by deleting FPS1 and over- expressing GLT1 in Saccharomyces cerevisiae.

To improve ethanol production in Saccharomyces cerevisiae, two yeast strains were constructed. In the mutant KAM-3, the FPS1 gene, which encodes a channel protein responsible for glycerol export, was deleted. The mutant KAM-11 had the GLT1 gene ( encoding glutamate synthase) placed under the PGK1 promoter while having the FPS1 deletion. Growth rate and biomass concentration remained virtually unchanged with the mutant KAM-11, compared to that of the parent. Over-expression of GLT1 by the PGK1 promoter along with FPS1 deletion resulted in a 14% higher ethanol production and a 30% lower glycerol formation compared to the parental strain under anaerobic fermentation conditions. Furthermore, acetate and pyruvic acid formation was also reduced in order for cells to maintain redox balance.[1]

References

  1. Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae. Kong, Q.X., Gu, J.G., Cao, L.M., Zhang, A.L., Chen, X., Zhao, X.M. Biotechnol. Lett. (2006) [Pubmed]
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