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Damjanac, M. et al.

Fluoro-Jade((R)) B staining as useful tool to identify activated microglia and astrocytes in a mouse transgenic model of Alzheimer's disease.

Fluoro-Jade((R)) B is known as a high affinity fluorescent marker for the localization of neuronal degeneration during acute neuronal distress. However, one study suggested that fluoro-Jade((R)) B stains reactive astroglia in the primate cerebral cortex. In this study, we analyzed the staining of fluoro-Jade((R)) B alone or combined with specific markers for detection of glial fibrillary acidic protein (GFAP) or activated CD68 microglia in the double APP(SL)/PS1 KI transgenic mice of Alzheimer's disease (AD), which display a massive neuronal loss in the CA1 region of the hippocampus. Our results showed that fluoro-Jade((R)) B did not stain normal and degenerating neurons in this double mouse transgenic model. Fluoro-Jade((R)) B was co-localized with Abeta in the core of amyloid deposits and in glia-like cells expressing Abeta. Furthermore, fluoro-Jade((R)) B was co-localized with CD68/macrosialin, a specific marker of activated microglia, and with GFAP for astrocytes in APP(SL)/PS1 KI transgenic mice of AD. Taken together, these findings showed that fluoro-Jade((R)) B can be used to label activated microglia and astrocytes which are abundant in the brain of these AD transgenic mice. It could stain degenerating neurons as a result of acute insult while it could label activated microglia and astrocytes during a chronic neuronal degenerative process such as AD for example.[1]

References

Fluoro-Jade((R)) B staining as useful tool to identify activated microglia and astrocytes in a mouse transgenic model of Alzheimer's disease. Damjanac, M., Bilan, A.R., Barrier, L., Pontcharraud, R., Anne, C., Hugon, J., Page, G. Brain Res. (2007) [Pubmed]

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