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Vilalta, A. et al.

Adrián Vilalta, Gretchen Jimenez, Denis Rusalov, Rodrick Planchon, Peggy Lalor, Kristin Carner, Jennifer A. Chaplin, Michael Komai, Marston Manthorpe, David C. Kaslow, Alain Rolland,

Vaccination with polymerase chain reaction-generated linear expression cassettes protects mice against lethal influenza A challenge.

The feasibility of a linear expression cassette (LEC)-based influenza A DNA vaccine was demonstrated in mice, using a lethal dose (LD90) of a mouse-adapted A/Hong Kong/8/68 (H3N2) influenza strain. LECs expressing hemagglutinin (HA) from either the homotypic H3N2 or the heterotypic H1N1 (A/Puerto Rico/8/34) influenza virus were produced by polymerase chain reaction and either phosphodiester- or phosphorothioate-modified oligonucleotide primers. Survival subsequent to lethal viral challenge was used as a primary end point; weight loss was the secondary end point. Survival and weight loss data showed that protection can be achieved in mice with 50 microg of phosphate-buffered saline-formulated LEC DNA or 2 microg of Vaxfectin-formulated LEC DNA. Survival correlated with neutralizing antibody titers (hemagglutination inhibition, HAI); titers obtained after vaccination with LEC were equivalent to those obtained with HA (H3N2) plasmid DNA control. Vaccination with heterotypic H1 HA-LEC DNA provided no protection against viral challenge.[1]

References

Vaccination with polymerase chain reaction-generated linear expression cassettes protects mice against lethal influenza A challenge. Vilalta, A., Jimenez, G., Rusalov, D., Planchon, R., Lalor, P., Carner, K., Chaplin, J.A., Komai, M., Manthorpe, M., Kaslow, D.C., Rolland, A. Hum. Gene Ther. (2007) [Pubmed]

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