The effect of magnesium on glycolysis of permeabilized Ehrlich ascites tumor cells.
We have previously observed that extracellular Mg2+ influences the phosphofructokinase ( PFK) activity of intact Ehrlich Ascites tumour cells (EATC). In this study we have investigated the mechanism by which Mg2+ modulates this key glycolytic enzyme in EATC made permeable to the cation by either digitonin or dextran sulphate. Results showed that when Mg2+ is freely permeable to the cytosol, the in vivo PFK activity, calculated as FDP/G6P ratio, is not increased as it is in intact cells. We also observed that in permeabilized cells Mg2+ determines the increase of glucose 6 phosphate (G6P), fructose 1,6 bisphosphate ( FDP) and lactate production. We hypothesize that extracellular Mg2+ regulates PFK and glycolysis in these neoplastic cells not by entering the cytosol but by a specific interaction with the plasma membrane.[1]References
- The effect of magnesium on glycolysis of permeabilized Ehrlich ascites tumor cells. Wolf, F.I., Bossi, D., Cittadini, A. Biochem. Biophys. Res. Commun. (1991) [Pubmed]
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