Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Edrington, T.S. et al.

Tom S. Edrington, Russell L. Farrow, Ken J. Genovese, Todd R. Callaway, Robin C. Anderson, David J. Nisbet,

Influence of exogenous melatonin on horizontal transfer of Escherichia coli O157:H7 in experimentally infected sheep.

The objective of the current research was to determine if exogenous melatonin would exert a "protective" effect on the gastrointestinal tract of sheep and prevent or reduce the horizontal transfer of Escherichia coli O157:H7 from experimentally infected to noninfected or "naïve" sheep. Sixteen cross-bred ewes were housed indoors and adapted to a high concentrate ration. Ewes were randomly assigned to one of four rooms and treatment (three ewes/room, six ewes/treatment) and received either control (gelatin capsule only) or melatonin (5.0 mg/kg body weight [BW]/d). Four additional ewes served as "carrier" sheep (one/room) and were experimentally infected via oral gavage with E. coli O157:H7. Three days post-challenge, carrier ewes were housed with naïve sheep and remained with them for the remainder of the experimental period. Treatments were administered to the naïve sheep 1 day prior to introduction of the carrier sheep and on each of the remaining 7 days of the experimental period. Fecal samples were collected via rectal palpation from the carrier sheep daily throughout experiment and from the naïve sheep daily for 5 days, starting 2 days following introduction of the carriers. On day 8 of the experiment, all ewes were euthanized and tissues from the rumen, ileum, cecum, colon, and rectum as well as their respective lumen contents collected. The carrier sheep quickly infected the naïve ewes, which had similar fecal concentrations as the carrier animals throughout the 5-day sampling period. Melatonin treatment had no effect (p > 0.10) on daily fecal shedding, luminal content concentrations, or in the percentage of gastrointestinal tract tissue positive for the inoculated strain of E. coli O157:H7.[1]

References

Influence of exogenous melatonin on horizontal transfer of Escherichia coli O157:H7 in experimentally infected sheep. Edrington, T.S., Farrow, R.L., Genovese, K.J., Callaway, T.R., Anderson, R.C., Nisbet, D.J. Foodborne Pathog. Dis. (2009) [Pubmed]

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