Generation and characterization of monoclonal antibodies against Staphylococcus aureus thermonuclease.
Monoclonal antibodies (MAbs) against Staphylococcus aureus thermonuclease ( TN) were raised by immunizing BALB/c mice with a commercial TN preparation. Six monoclones were generated producing MAbs specific for S. aureus TN as tested in Western blots and ELISA. They all combined with a 17 kD and a 21 kD protein, respectively, both of which showed DNase activity. All MAbs were of IgG1 isotype with kappa light chain. Competition ELISA showed that five of the MAbs recognized a total of three different binding sites of TN, designated I, II and III, respectively. Only the anti-site II MAbs inhibited the DNase activity. A MAb-based sandwich ELISA showed a lower detection limit for TN of approximately 0.5 ng/ ml protein. Only S. aureus strains (culture supernatants) showed positive ELISA (31 positive/31 tested), although other tested gram positive cocci produced thermostable nucleases. The MAbs have potentials as reagents for rapid and specific detection of S. aureus.[1]References
- Generation and characterization of monoclonal antibodies against Staphylococcus aureus thermonuclease. Brakstad, O.G., Maeland, J.A. APMIS (1989) [Pubmed]
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