Disease relevance of nucI

• Staphylococcal enterotoxin and thermonuclease production during induced bovine mastitis and the clinical reaction of enterotoxin in udders [1].
• Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius [2].
• An aliquot (0.1 ml) of the positive blood culture, which showed growth of gram-positive cocci and demonstrated thermonuclease activity, was inoculated into the module well of a Bactometer incubator (bioMerieux Vitek, Hazelwood, Mo.) containing 0.6 ml of Mueller-Hinton agar supplemented with oxacillin (2 microg/ml) [3].
• These are enterotoxin B, leukotoxin D, enterotoxin, serin proteases (SplA and SplC), thermonuclease, an IgG binding protein and two so far unknown proteins in S. aureus with similarities to SceD precursor in Staphylococcus carnosus and to synergohymenotropic toxin precursor in Streptococcus intermedius [4].

High impact information on nucI

• Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius [5].
• Production of staphylococcal enterotoxins C1 and C2 and thermonuclease throughout the growth cycle [6].
• Synthesis of enterotoxins C1 and C2 and thermonuclease throughout the growth cycle was investigated with Staphylococcus aureus type strains FRI137 and FRI361 and S. aureus isolates M5 (C1) and L2 (C2) of animal origin [6].
• Discrepancies between agglutination and tube coagulase identifications were resolved by use of the thermonuclease, mannitol fermentation, and slide coagulase tests [7].
• Simultaneous testing for clumping factor, coagulase, deoxyribonuclease, and thermonuclease was performed on 189 clinical isolates of gram-positive cocci with strong catalase activity to determine the suitability of the thermonuclease test as a routine procedure for the identification of Staphylococcus aureus [8].

Chemical compound and disease context of nucI

• A total of 445 blood cultures containing Gram-positive cocci in clusters were tested for the presence of Staphylococcus aureus with the Accuprobe, heat-stable thermonuclease, and latex agglutination using Staphaurex [9].
• The aim of this work was to study the activity of N. cataria extract on 44 Staphylococcus aureus strains, some resistant to methicillin, and S. aureus 6538P (American Type Culture Collection) by evaluating the effect of subminimum inhibitory concentrations on coagulase, DNAse, thermonuclease and lipase production, and on in-vitro adherence [10].
• An overlay of toluidine blue agar was added and the plates reincubated for 4 h at 37 degrees C. The formation of thermonuclease correlated with the formation of coagulase, and the results indicated that Staphylococcus aureus could be present in swimming pool water without the presence of either coliform or faecal coliform bacteria [11].

Biological context of nucI

• The cell count determined in a sample of sausage had no definite correlation with the thermonuclease activity of the sample [12].
• Competition ELISA showed that five of the MAbs recognized a total of three different binding sites of TN, designated I, II and III, respectively [13].
• Blood cultures submitted to the Clinical Microbiology Laboratory, Queen Alia Military Hospital, Amman during 1999-2001 were examined to evaluate thermonuclease testing for identifying Staphylococcus aureus in blood culture broths growing gram-positive cocci [14].

Anatomical context of nucI

• Enterotoxigenicity correlated well with the following characteristics: pigment production, presence of the clumping factor, coagulation of rabbit plasma, haemolysis of sheep erythrocytes, positivity in the STAPHYtest, fermentation of mannitol, strong haemolysis of bovine blood and strong thermonuclease activity [15].

Associations of nucI with chemical compounds

• Furthermore, strain FRI137 produced thermonuclease earlier and at lower cell populations than it did enterotoxin C1 [6].
• Koupal-Deibel deoxyribonucleic acid agar was somewhat superior to toluidine blue deoxyribonucleic acid agar for thermonuclease assay of fermented sausage casings [16].
• Bovine and caprine isolates were 34.1 and 25% thermonuclease-positive, respectively, while ovine isolates were only 14.2% thermonuclease-positive [17].
• The widely promulgated interpretation that all degrees of clotting in coagulase plasma are a positive identification of S. auerus was disproved by the use of other tests such as anaerobic glucose fermentation, thermonuclease production, and lysostaphin sensitivity [18].

Analytical, diagnostic and therapeutic context of nucI

• Detection of enterotoxigenic Staphylococcus aureus in dried skimmed milk: use of the polymerase chain reaction for amplification and detection of staphylococcal enterotoxin genes entB and entC1 and the thermonuclease gene nuc [19].
• Immunologic tests have shown varied sensitivities, the thermonuclease test, while sensitive, is not practical for routine use, and probe tests are expensive [20].
• The thermonuclease test is well suited for use as a primary clinical laboratory procedure for the identification of Staphylococcus aureus [8].
• Six monoclones were generated producing MAbs specific for S. aureus TN as tested in Western blots and ELISA [13].
• In Isosensitest, brain-heart infusion, tryptic soy, nutrient and gas-liquid chromatography broths (all of which do not contain liquoid) thermonuclease detection was uncomplicated [21].

References