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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Primary sequence and developmental expression of a novel Drosophila melanogaster src gene.

We have sequenced a cDNA clone for the Drosophila melanogaster gene Dsrc28C, a homolog of the vertebrate gene c-src. The cDNA contains a single open reading frame encoding a protein of 66 kilodaltons which contains features highly conserved within the src family of tyrosine protein kinases. Novel structural features of the Dsrc28C protein include a basic pI and a polyglycine domain near the amino terminus. Cell-free translation of in vitro-transcribed RNA yielded a protein of the predicted size which could be immunoprecipitated by anti-v-src antisera. RNA blot hybridization revealed that the gene is expressed predominantly during embryogenesis, in imaginal disks of third-instar larvae, and in adult females. In situ hybridization showed that expression in adult females is largely confined to nurse cells and developing oocytes.[1]


  1. Primary sequence and developmental expression of a novel Drosophila melanogaster src gene. Gregory, R.J., Kammermeyer, K.L., Vincent, W.S., Wadsworth, S.G. Mol. Cell. Biol. (1987) [Pubmed]
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