High impact information on Btk29A

• Drosophila ring canal growth requires Src and Tec kinases [1].
• The 66-kD protein was shown to represent the cell periphery-associated form of the protein through antibody staining of larval salivary glands expressing a heat shock promoter-driven, full-length Dsrc28C cDNA [2].
• Indirect immunofluorescence microscopy reveals that Dsrc28C protein is localized to the cell periphery during cellular blastoderm and gastrulation [2].
• Tec29 encodes the only known Drosophila member of the Tec tyrosine kinases [3].
• Loss of Tec29 function in the female germline results in a phenotype strikingly similar to that caused by the loss of Src64 function [4].

Biological context of Btk29A

• While Btk29A appears to be regulated by Pcs during its early role in patterning and segmentation, it does not appear to be a major target of Pcs regulation during muscle development [5].
• Enhanced phosphorylation and enzymatic activity of phosphoglucomutase by the Btk29A tyrosine kinase in Drosophila [6].
• The Tec29 tyrosine kinase is required during Drosophila embryogenesis and interacts with Src64 in ring canal development [3].
• We show that mutations affecting Tec29, an essential gene encoding a member of the Tec family of protein tyrosine kinases, dominantly enhance the Src64 ring canal phenotype [4].
• Here, we show that Tec29 localizes to ring canals via its Src homology 3 (SH3) and Src homology 2 (SH2) domains [7].

Anatomical context of Btk29A

• Tec29 mutant egg chambers are defective in transfer of cytoplasm from the accessory nurse cells through the ring canals into the oocyte [3].
• Tec29 controls actin remodeling and endoreplication during invagination of the Drosophila embryonic salivary glands [8].

Associations of Btk29A with chemical compounds

• The Drosophila Btk29A tyrosine kinase is suggested to be involved in diverse processes, although its target proteins are unknown [6].
• Tec29 activity is required for its own ring canal localization, suggesting that a phosphotyrosine ligand for the SH2 domain is generated by Tec29 itself [7].
• Novel structural features of the Dsrc28C protein include a basic pI and a polyglycine domain near the amino terminus [9].

Enzymatic interactions of Btk29A

• Src64 regulates this process by phosphorylating Y677 within the kinase domain of Tec29, an event required for Tec29 activation [7].

Regulatory relationships of Btk29A

• In addition, we show that a mutation in Tec29A partially suppresses a gain-of-function mutation in tor [10].

Other interactions of Btk29A

• Embryos mutant for Tec29, the Drosophila Tec family tyrosine kinase, showed delayed invagination of the salivary placodes [8].

References