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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Equivalence of UDS responses for established cell lines and primary cells derived from the mei-9a and mus201D1 excision repair-deficient strains of Drosophila melanogaster.

Autoradiographic analysis of unscheduled DNA synthesis in response to DNA damage produced by relatively high doses of the direct-acting, monofunctional alkylating agent, MMS, (4.5 mM) and UV (40 J/m2) demonstrates that established cell lines, derived from the mei-9a and mus201D1 excision repair-deficient strains of Drosophila melanogaster, perform no measurable incorporation of [3H]thymidine into repair patches, in accordance with the observations made for the corresponding primary embryonic cells derived from the two mutagen-sensitive strains of flies. These established cell lines can therefore be used as appropriate models for both the examination of the biochemical basis of the genetic defects in the mei-9 and mus201 mutations and the role of excision-repair processes in spontaneous and induced mutation induction in eukaryotic cells.[1]

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