Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions.
By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of temperature-resistant derivatives of the lysogen resulted in a fusion of the lac genes to a region of deoxyribonucleic acid that is transcribed under the control of the ilvC regulatory elements. A strain bearing the fusion was shown to be inducible for beta-galactosidase by acetohydroxybutyrate, a natural inducer of acetohydroxy acid isomeroreductase. Induction of the lysogen by mitomycin C led to the isolation of a plaque-forming lambda derivative carrying this ilvC-lac fusion.[1]References
- Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions. Smith, J.M., Umbarger, H.E. J. Bacteriol. (1977) [Pubmed]
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