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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

lacZ  -  beta-D-galactosidase

Escherichia coli CFT073

 
 
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Disease relevance of lacZ

 

Psychiatry related information on lacZ

  • This system has several advantages compared with the conventional umu test: drastic reduction of the sample volume, less time-consuming for beta-galactosidase detection (free from substrate reaction time) and lower detection limit for the three mutagens (AF-2, MMC, 2-AA) [5].
 

High impact information on lacZ

 

Chemical compound and disease context of lacZ

 

Biological context of lacZ

 

Anatomical context of lacZ

 

Associations of lacZ with chemical compounds

  • Strain NU229 contained a Fur-repressed fusion based on derepression of lacZ expression in its manganese-resistant derivative [25].
  • This à la carte host thus translated the conversion of gamma-HCH to TCB into upregulation of lacZ [26].
  • New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions [27].
  • This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3' end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy [28].
  • The patterns of frameshift mutagenicity in the lacZ assay are similar to those in an assay based on the reversion of mutations in the tetracycline-resistance gene of the plasmid pBR322 [29].
 

Regulatory relationships of lacZ

  • We constructed B. subtilis mutants and showed that the increased beta-galactosidase activity generated in response to the addition of galactan was eliminated by inactivating lacA or galA but unaffected by the inactivation of yesZ [30].
  • Nine lacZ fusion strains which expressed beta-galactosidase after growth under sulfate starvation conditions but not after growth in the presence of sulfate were found [31].
 

Other interactions of lacZ

 

Analytical, diagnostic and therapeutic context of lacZ

  • To examine lacZ expression in transduced hepatocytes in vivo, we used a mouse hepatocyte transplantation system [3].
  • A multiplex quantitative PCR assay was used to measure photoproduct formation and repair at the gene level in both the constitutive lacI gene and the inducible lacZ gene, simultaneously [36].
  • Adenovirus vectors carrying the E. coli lacZ gene (Ad.RSV.beta-gal) and the firefly luciferase gene (AdCMV-luc) as reporters were administered to the liver of adult Wistar rats, either via infusion into the portal vein (intraportal infusion; IPI) or via perfusion of the vascularity isolated liver (isolated liver perfusion; ILP) [37].
  • Electrophoretic mobility shift assay showed strong binding of M. smegmatis RNA polymerase holoenzyme to the 400-bp fragment that expresses lacZ in mycobacterial species and a weak binding to the 280-bp fragment that expresses only in E. coli DH5alpha [38].
  • The performance of electroporation was evaluated using pJB3 (4.8 kb), pBI121 (12.8 kb) and pFAJ31.2 (24 kb) plasmid DNAs containing selectable (Ap, Km, Tc) and screenable (gusA, lacZ) marker genes [39].

References

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