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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

lacZ  -  beta-D-galactosidase

Escherichia coli CFT073

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Disease relevance of lacZ


Psychiatry related information on lacZ

  • This system has several advantages compared with the conventional umu test: drastic reduction of the sample volume, less time-consuming for beta-galactosidase detection (free from substrate reaction time) and lower detection limit for the three mutagens (AF-2, MMC, 2-AA) [5].

High impact information on lacZ


Chemical compound and disease context of lacZ


Biological context of lacZ


Anatomical context of lacZ


Associations of lacZ with chemical compounds

  • Strain NU229 contained a Fur-repressed fusion based on derepression of lacZ expression in its manganese-resistant derivative [25].
  • This à la carte host thus translated the conversion of gamma-HCH to TCB into upregulation of lacZ [26].
  • New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions [27].
  • This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3' end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy [28].
  • The patterns of frameshift mutagenicity in the lacZ assay are similar to those in an assay based on the reversion of mutations in the tetracycline-resistance gene of the plasmid pBR322 [29].

Regulatory relationships of lacZ

  • We constructed B. subtilis mutants and showed that the increased beta-galactosidase activity generated in response to the addition of galactan was eliminated by inactivating lacA or galA but unaffected by the inactivation of yesZ [30].
  • Nine lacZ fusion strains which expressed beta-galactosidase after growth under sulfate starvation conditions but not after growth in the presence of sulfate were found [31].

Other interactions of lacZ


Analytical, diagnostic and therapeutic context of lacZ

  • To examine lacZ expression in transduced hepatocytes in vivo, we used a mouse hepatocyte transplantation system [3].
  • A multiplex quantitative PCR assay was used to measure photoproduct formation and repair at the gene level in both the constitutive lacI gene and the inducible lacZ gene, simultaneously [36].
  • Adenovirus vectors carrying the E. coli lacZ gene (Ad.RSV.beta-gal) and the firefly luciferase gene (AdCMV-luc) as reporters were administered to the liver of adult Wistar rats, either via infusion into the portal vein (intraportal infusion; IPI) or via perfusion of the vascularity isolated liver (isolated liver perfusion; ILP) [37].
  • Electrophoretic mobility shift assay showed strong binding of M. smegmatis RNA polymerase holoenzyme to the 400-bp fragment that expresses lacZ in mycobacterial species and a weak binding to the 280-bp fragment that expresses only in E. coli DH5alpha [38].
  • The performance of electroporation was evaluated using pJB3 (4.8 kb), pBI121 (12.8 kb) and pFAJ31.2 (24 kb) plasmid DNAs containing selectable (Ap, Km, Tc) and screenable (gusA, lacZ) marker genes [39].


  1. Characterization of ferric and ferrous iron transport systems in Vibrio cholerae. Wyckoff, E.E., Mey, A.R., Leimbach, A., Fisher, C.F., Payne, S.M. J. Bacteriol. (2006) [Pubmed]
  2. Cloning and characterization of a beta-galactosidase encoding region in Lactobacillus coryniformis CECT 5711. Corral, J.M., Ba??uelos, O., Adrio, J.L., Velasco, J. Appl. Microbiol. Biotechnol. (2006) [Pubmed]
  3. Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector. Lu, B., Gupta, S., Federoff, H. Hepatology (1995) [Pubmed]
  4. High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Nishi, T., Yoshizato, K., Yamashiro, S., Takeshima, H., Sato, K., Hamada, K., Kitamura, I., Yoshimura, T., Saya, H., Kuratsu, J., Ushio, Y. Cancer Res. (1996) [Pubmed]
  5. Electrochemical mutagen screening using microbial chip. Matsui, N., Kaya, T., Nagamine, K., Yasukawa, T., Shiku, H., Matsue, T. Biosensors & bioelectronics. (2006) [Pubmed]
  6. Gene therapy of metastatic cancer by in vivo retroviral gene targeting. Hurford, R.K., Dranoff, G., Mulligan, R.C., Tepper, R.I. Nat. Genet. (1995) [Pubmed]
  7. Three-dimensional structure of beta-galactosidase from E. coli. Jacobson, R.H., Zhang, X.J., DuBose, R.F., Matthews, B.W. Nature (1994) [Pubmed]
  8. Whole animal cell sorting of Drosophila embryos. Krasnow, M.A., Cumberledge, S., Manning, G., Herzenberg, L.A., Nolan, G.P. Science (1991) [Pubmed]
  9. Translational induction of heat shock transcription factor sigma32: evidence for a built-in RNA thermosensor. Morita, M.T., Tanaka, Y., Kodama, T.S., Kyogoku, Y., Yanagi, H., Yura, T. Genes Dev. (1999) [Pubmed]
  10. Construction and characterization of a triple-recombinant vaccinia virus encoding B7-1, interleukin 12, and a model tumor antigen. Carroll, M.W., Overwijk, W.W., Surman, D.R., Tsung, K., Moss, B., Restifo, N.P. J. Natl. Cancer Inst. (1998) [Pubmed]
  11. Neurotrophin-3 transduction attenuates cisplatin spiral ganglion neuron ototoxicity in the cochlea. Bowers, W.J., Chen, X., Guo, H., Frisina, D.R., Federoff, H.J., Frisina, R.D. Mol. Ther. (2002) [Pubmed]
  12. Efficacy of cationic liposome-mediated gene transfer to mesangial cells in vitro and in vivo. Madry, H., Reszka, R., Bohlender, J., Wagner, J. J. Mol. Med. (2001) [Pubmed]
  13. The bzd gene cluster, coding for anaerobic benzoate catabolism, in Azoarcus sp. strain CIB. López Barragán, M.J., Carmona, M., Zamarro, M.T., Thiele, B., Boll, M., Fuchs, G., García, J.L., Díaz, E. J. Bacteriol. (2004) [Pubmed]
  14. Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding an NRAMP-like Mn(2+) transporter in Escherichia coli. Patzer, S.I., Hantke, K. J. Bacteriol. (2001) [Pubmed]
  15. Neural toxicity of retroviruses. Hurley, M.J., Bradford, H.F., O'Hare, K., Stern, G.M., Uchida, K. Neurosci. Lett. (1996) [Pubmed]
  16. Characterization of the fatty acid-responsive transcription factor FadR. Biochemical and genetic analyses of the native conformation and functional domains. Raman, N., Black, P.N., DiRusso, C.C. J. Biol. Chem. (1997) [Pubmed]
  17. An activator of glutamate decarboxylase genes regulates the expression of enteropathogenic Escherichia coli virulence genes through control of the plasmid-encoded regulator, Per. Shin, S., Castanie-Cornet, M.P., Foster, J.W., Crawford, J.A., Brinkley, C., Kaper, J.B. Mol. Microbiol. (2001) [Pubmed]
  18. The molybdate-responsive Escherichia coli ModE transcriptional regulator coordinates periplasmic nitrate reductase (napFDAGHBC) operon expression with nitrate and molybdate availability. McNicholas, P.M., Gunsalus, R.P. J. Bacteriol. (2002) [Pubmed]
  19. Impact of antibiotic stress on acid and heat tolerance and virulence factor expression of Escherichia coli O157:H7. Azizoglu, R.O., Drake, M. J. Food Prot. (2007) [Pubmed]
  20. Characterization of pepR1, a gene coding for a potential transcriptional regulator of Lactobacillus delbrueckii subsp. lactis DSM7290. Stucky, K., Schick, J., Klein, J.R., Henrich, B., Plapp, R. FEMS Microbiol. Lett. (1996) [Pubmed]
  21. The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation. Iost, I., Dreyfus, M. EMBO J. (1995) [Pubmed]
  22. Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs. Bragonzi, A., Boletta, A., Biffi, A., Muggia, A., Sersale, G., Cheng, S.H., Bordignon, C., Assael, B.M., Conese, M. Gene Ther. (1999) [Pubmed]
  23. Adenovirus-mediated suicide gene transduction: feasibility in lens epithelium and in prevention of posterior capsule opacification in rabbits. Malecaze, F., Couderc, B., de Neuville, S., Serres, B., Mallet, J., Douin-Echinard, V., Manenti, S., Revah, F., Darbon, J.M. Hum. Gene Ther. (1999) [Pubmed]
  24. Enhanced repair of articular cartilage defects in vivo by transplanted chondrocytes overexpressing insulin-like growth factor I (IGF-I). Madry, H., Kaul, G., Cucchiarini, M., Stein, U., Zurakowski, D., Remberger, K., Menger, M.D., Kohn, D., Trippel, S.B. Gene Ther. (2005) [Pubmed]
  25. An iron- and fur-repressed Legionella pneumophila gene that promotes intracellular infection and encodes a protein with similarity to the Escherichia coli aerobactin synthetases. Hickey, E.K., Cianciotto, N.P. Infect. Immun. (1997) [Pubmed]
  26. Surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (gamma-hexachlorocyclohexane) into lacZ-based phenotypes. Mohn, W.W., Garmendia, J., Galvao, T.C., de Lorenzo, V. Environ. Microbiol. (2006) [Pubmed]
  27. New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Becker, A., Schmidt, M., Jäger, W., Pühler, A. Gene (1995) [Pubmed]
  28. Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli. Tøndervik, A., Torgersen, H.R., Botnmark, H.K., Strøm, A.R. J. Bacteriol. (2006) [Pubmed]
  29. Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: potency of responses and relationship to slipped mispairing models. Hoffmann, G.R., Calciano, M.A., Lawless, B.M., Mahoney, K.M. Environ. Mol. Mutagen. (2003) [Pubmed]
  30. Bioinformatic, Genetic, and Biochemical Evidence that Some Glycoside Hydrolase Family 42 {beta}-Galactosidases Are Arabinogalactan Type I Oligomer Hydrolases. Shipkowski, S., Brenchley, J.E. Appl. Environ. Microbiol. (2006) [Pubmed]
  31. Identification of sulfate starvation-regulated genes in Escherichia coli: a gene cluster involved in the utilization of taurine as a sulfur source. van der Ploeg, J.R., Weiss, M.A., Saller, E., Nashimoto, H., Saito, N., Kertesz, M.A., Leisinger, T. J. Bacteriol. (1996) [Pubmed]
  32. Alp suppression of Lon: dependence on the slpA gene. Trempy, J.E., Kirby, J.E., Gottesman, S. J. Bacteriol. (1994) [Pubmed]
  33. Monitoring aromatic hydrocarbons by whole cell electrochemical biosensors. Paitan, Y., Biran, I., Shechter, N., Biran, D., Rishpon, J., Ron, E.Z. Anal. Biochem. (2004) [Pubmed]
  34. Increased recombinant protein production in Escherichia coli strains with overexpressed water-forming NADH oxidase and a deleted ArcA regulatory protein. Vemuri, G.N., Eiteman, M.A., Altman, E. Biotechnol. Bioeng. (2006) [Pubmed]
  35. Down-regulation of the kps region 1 capsular assembly operon following attachment of Escherichia coli type 1 fimbriae to D-mannose receptors. Schwan, W.R., Beck, M.T., Hultgren, S.J., Pinkner, J., Woolever, N.L., Larson, T. Infect. Immun. (2005) [Pubmed]
  36. High resolution mapping of UV-induced photoproducts in the Escherichia coli lacI gene. Inefficient repair of the non-transcribed strand correlates with high mutation frequency. Chandrasekhar, D., Van Houten, B. J. Mol. Biol. (1994) [Pubmed]
  37. Isolated-organ perfusion for local gene delivery: efficient adenovirus-mediated gene transfer into the liver. de Roos, W.K., Fallaux, F.J., Marinelli, A.W., Lazaris-Karatzas, A., von Geusau, A.B., van der Eb, M.M., Cramer, S.J., Terpstra, O.T., Hoeben, R.C. Gene Ther. (1997) [Pubmed]
  38. Identification of a novel mycobacterial transcriptional regulator and its involvement in growth rate dependence and stringent control. Kamalakannan, V., Ramachandran, G., Narayanan, S., Vasan, S.K., Narayanan, P.R. FEMS Microbiol. Lett. (2002) [Pubmed]
  39. Stable electrotransformation of symbiont candidate diazotrophic bacterium with plasmids carrying selectable and screenable marker genes. Korányi, P., Burg, K., Berényi, M. Res. Microbiol. (1998) [Pubmed]
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