Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Udupa, K.B. et al.

Proerythroblast stimulating activity: its purification from mouse serum and its effect on mouse erythroid cell proliferation in vitro.

A factor in mouse serum which stimulates proerythroblast proliferation in in vitro culture (proerythroblast stimulating activity-PSA) was purified by green A dye ligand and high performance liquid chromatography (HPLC). As judged by HPLC, PSA obtained after these two steps appeared to be a homogenous protein of Mr 100,000. It increased the proliferation of proerythroblasts when added alone to a liquid culture of bone marrow which was treated with anti-RBC antibody to remove haemoglobin containing erythroid cells. PSA functioned synergistically with erythropoietin ( Ep) so that when added to culture together proliferation was 10-fold higher than when added to culture alone. PSA also increased CFU-E number but only when added together with Ep. Dose-response studies indicated that PSA increased CFU-E number when Ep remained constant and vice versa. PSA addition to culture could be delayed by as much as 12 h without any decrease in the number of CFU-E colonies that developed. When PSA was added to BFU-E cultures at the time of culture initiation no increase in BFU-E was seen. If, however, PSA was added 5 d after culture initiation BFU-E colonies were significantly increased. These results demonstrate that there is a factor ( PSA) which can be purified from mouse serum which is not Ep. Its major site of action appears to be the more mature erythroid precursors that have the capacity to divide.[1]

References

Proerythroblast stimulating activity: its purification from mouse serum and its effect on mouse erythroid cell proliferation in vitro. Udupa, K.B., Lipschitz, D.A. Br. J. Haematol. (1988) [Pubmed]

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