The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

About

Terms

 

 
 
 
 

Protein redistribution in model membranes: clearing of M13 coat protein from calcium-induced gel-phase regions in phosphatidylserine/phosphatidylcholine multilamellar vesicles.

A model system of M13 bacteriophage coat protein reconstituted into multilamellar vesicles composed of phosphatidylserine (PS) and phosphatidylcholine (PC) is used to examine protein redistribution in membranes in the presence of Ca2+. The reconstitution procedure is analyzed by using protease digestion and gel permeation chromatography of radioactively labeled coat protein and is found to incorporate coat protein into lipid vesicles predominantly in the in vivo orientation and without aggregation. Quenching of protein tryptophanyl fluorescence by spin-labeled PC is used to determine the local lipid environment of the coat protein in binary lipid mixtures. The distribution of coat protein between fluid liquid-crystal (LC) and Ca2+-induced gel (G) phases in PS/PC multilayers, expressed as a concentration ratio RLC/G, is found to be 25 +/- 5 in favor of the fluid phase, indicating significant clearing of membrane protein from Ca2+-induced gel-phase regions.[1]

References

  1. Protein redistribution in model membranes: clearing of M13 coat protein from calcium-induced gel-phase regions in phosphatidylserine/phosphatidylcholine multilamellar vesicles. Florine, K.I., Feigenson, G.W. Biochemistry (1987) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities