The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Permeability of reconstituted sarcoplasmic reticulum vesicles. Reconstitution of the K+, Na+ channel.

Permeability properties of reconstituted rabbit skeletal muscle sarcoplasmic reticulum vesicles were characterized by measuring efflux rates of [3H]inulin, [3H]choline+, 86Rb+, and 22Na+, as well as membrane potential changes using the voltage-sensitive probe, 3,3'-dipentyl-2,2'-oxacarbocyanine. Native vesicles were dissociated with deoxycholate and were reconstituted by dialysis. Energized Ca2+ accumulation was partially restored. About 1/2 of the reconstituted vesicles were found to be 'leaky', i.e., permeable to choline+ of Tris+ but not to inulin. The remaining reconstituted vesicles were 'sealed', i.e., impermeable to choline+, Tris+ and inulin. Sealed reconstituted vesicles could be further subdivided according to their K+, Na+ permeability. About 1/2, previously designated Type I, were readily permeable to K+ and Na+, indicating the presence of the K+, Na+ channel of sarcoplasmic reticulum. The remaining sealed vesicles (Type II) formed a permeability barrier to K+ and Na+, suggesting that they lacked the K+, Na+ channel. These studies show that the K+, Na+ channel of sarcoplasmic reticulum can be solubilized with detergent and reconstituted with retention of activity. Furthermore, our results suggest that part or all of the decreased Ca2+-loading efficiency of reconstituted vesicles may be due to the presence of a significant fraction of leaky vesicles.[1]

References

  1. Permeability of reconstituted sarcoplasmic reticulum vesicles. Reconstitution of the K+, Na+ channel. Young, R.C., Allen, R., Meissner, G. Biochim. Biophys. Acta (1981) [Pubmed]
 
WikiGenes - Universities