Disease relevance of Dioc(5)(3)

• We propose that a virion will arrive within a typical median time at such a site, generating a membrane signal such as an increased membrane fluorescence in cells labeled with the voltage-sensitive dyes 8-anilino-1-naphthalene-sulfonate (Mg-salt) (ANS), N-phenylnaphthylamine (NPA), or 3,3'-dipentyl-2,2'-oxacarbocyanine (di-O-C5[3]) [1].
• In an effort to improve antibiotic susceptibility testing, we developed a flow cytometric technique for Staphylococcus aureus in which antibiotic action is determined by monitoring drug-induced membrane potential changes, using the potential-sensitive dye 3,3'-dipentyloxacarbocyanine iodide [2].

High impact information on Dioc(5)(3)

• Results indicate that divalent fragments of anti-Fab antibodies mitogenic for B cells stimulate membrane depolarization detectable by cytofluorometric analysis of 3,3'-dipentyloxacarbocyanine iodide-stained cells [3].
• Membrane potentials were measured using the voltage-sensitive fluorescent dye 3,3'-dipentyl-2,2'-oxacarbocyanine [4].
• The aim of the present study was to see the correlation between changes in membrane potential and clonogenic ability of HeLa cells after heat treatment of 41-44 degrees C. Membrane potential was measured by the fluorescence polarization of the plasma membrane probe 3,3'-dipentyloxacarbocyanine by flow cytometry [5].
• Crude venoms of three poisonous jellyfish produce membrane depolarization as determined by the loss of fluorescence intensity of 3,3'-dipentyloxacarbocyanine iodide loaded cells measured by flow cytometry [6].
• With 200 microM tBOOH, the rate of decrease in membrane potential, measured by 3,3'-dipentyloxacarbocyanine iodide fluorescence, inversely correlated with glutathione peroxidase [7].

Anatomical context of Dioc(5)(3)

• The effect of elevated temperature on transmembrane potential was studied in Chinese hamster ovary cells in vitro using tetraphenylphosphonium cation (TPP+) and 3,3'-dipentyloxacarbocyanine [Di-O-C5(3)], two unrelated lipophilic cation probes that equilibrate across the plasma membrane according to the transmembrane potential [8].
• These data indicate that various neutrophil functions may be regulated independently at other than the receptor level and that neutrophil chemotactic responses to FMLP and C5a do not appear to be dependent on membrane events registered by 3,3'-dipentyloxacarbocyanine [9].
• Permeability properties of reconstituted rabbit skeletal muscle sarcoplasmic reticulum vesicles were characterized by measuring efflux rates of [3H]inulin, [3H]choline+, 86Rb+, and 22Na+, as well as membrane potential changes using the voltage-sensitive probe, 3,3'-dipentyl-2,2'-oxacarbocyanine [10].

Analytical, diagnostic and therapeutic context of Dioc(5)(3)

• In order to determine the nature of the dose dependence of depolarization (a measure of PMN activation), PMNs equilibrated with the fluorescent probe 3,3'-dipentyloxacarbocyanine were analyzed by flow cytometry [11].

References