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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Structural organization and expression of human DNA sequences related to the transforming gene of avian myeloblastosis virus.

Bacteriophage libraries of human DNA were screened for sequences homologous to the transforming gene (v-myb) of avian myeloblastosis virus. The three overlapping clones isolated were shown to contain a total of 1.0 kilobase pair (kbp) of sequence related to v-myb distributed over 6.2 kbp. Restriction enzyme mapping and heteroduplex analysis revealed the presence of five myb-related domains interrupted by four stretches of non-homology. To study the extent of human DNA coding sequences that constitute the myb gene homologue, c-myb (human), probes spanning about 30 kbp were prepared from the clones and used to study transcription in a human hematopoietic cell line (MOLT-4). Each of the probes hybridized a 4.5-kilobase transcript, which suggests that either the c-myb (human) gene encompasses 30 kbp or it contains two or more transcription units that each give rise to a mRNA of 4.5 kilobases.[1]

References

  1. Structural organization and expression of human DNA sequences related to the transforming gene of avian myeloblastosis virus. Franchini, G., Wong-Staal, F., Baluda, M.A., Lengel, C., Tronick, S.R. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
 
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