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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A two-site model for the esterase and dehydrogenase activities of sheep liver aldehyde dehydrogenase.

Although aldehyde dehydrogenase (ALDH) from sheep liver cytosol has a broad specificity, it will not oxidize the aldehyde group of glyoxylic acid which is in fact an inhibitor of the enzyme. The inhibition pattern is non-linear but competitive at high propionaldehyde concentrations (2-20 mM); however, a simple non-competitive pattern is observed at low (less than 100 microM) propionaldehyde concentrations (Ki = 1.6 mM). The esterase activity was unaffected by glyoxylic acid in the absence of NAD+ but a simple competitive inhibition pattern (Ki = 2.5 mM) was observed with respect to 4-nitrophenyl acetate in the presence of NAD+. The data require a two-site model in which ester and aldehyde binding sites are distinct but with a second propionaldehyde molecule, and glyoxylic acid, binding at or near the ester binding site. Consistent with this model is the fact that chloral hydrate was a non-competitive inhibitor of the esterase activity in the presence of NAD+ but a competitive inhibitor in its absence. The enzyme exhibited hysteretic behavior governed by the protonated form of an ionizable group with an apparent pKa of 7.55.[1]


  1. A two-site model for the esterase and dehydrogenase activities of sheep liver aldehyde dehydrogenase. Blackwell, L.F., Bennett, A.F., Crow, K.E., Buckley, P.D., Deady, L.W. Pharmacol. Biochem. Behav. (1983) [Pubmed]
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