High impact information on LOC443343

• Selective alteration of the rate-limiting step in cytosolic aldehyde dehydrogenase through random mutagenesis [1].
• Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate [2].
• 1. p-Nitrophenyl (PNP) acetate and propionate show a burst of p-nitrophenoxide release when their hydrolysis is catalysed by sheep liver cytosolic aldehyde dehydrogenase [3].
• Probing the active site of cytoplasmic aldehyde dehydrogenase with a chromophoric reporter group [4].
• Involvement of serine 74 in the enzyme-coenzyme interaction of rat liver mitochondrial aldehyde dehydrogenase [5].

Biological context of LOC443343

• The kinetics of the NAD+-dependent oxidation of aldehydes, catalysed by aldehyde dehydrogenase purified from sheep liver mitochondria, were studied in detail [6].
• Aldehyde dehydrogenase is able to catalyse the hydrolysis of p-nitrophenyl esters [6].
• Data indicated 93% concordance of leukaemia inhibitory factor with aldehyde dehydrogenase 2 located on bovine chromosome 17, and concordance of 81% with myelin basic protein situated on bovine chromosome 24 [7].

Anatomical context of LOC443343

• Kinetic properties of aldehyde dehydrogenase from sheep liver mitochondria [6].
• Several studies have shown preparations of the cytosolic aldehyde dehydrogenase (EC 1.2.1.3) from sheep and human liver and from human erythrocytes to exhibit complex kinetic behaviour in which the dependence of the initial velocity on the concentration of acetaldehyde gives rise to downwardly curving double-reciprocal plots [8].
• Although aldehyde dehydrogenase (ALDH) from sheep liver cytosol has a broad specificity, it will not oxidize the aldehyde group of glyoxylic acid which is in fact an inhibitor of the enzyme [9].

Associations of LOC443343 with chemical compounds

• Mechanism of inactivation of sheep liver cytoplasmic aldehyde dehydrogenase by disulfiram [10].
• Studies on the mechanism of sheep liver cytosolic aldehyde dehydrogenase. The effect of pH on the aldehyde binding reactions and a re-examination of the problem of the site of proton release in the mechanism [11].
• The activation of aldehyde dehydrogenase by diethylstilboestrol and 2,2'-dithiodipyridine [12].
• Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate [13].
• The kinetic properties of highly purified preparations of sheep liver cytoplasmic aldehyde dehydrogenase (preparations that had been shown to be free from contamination with the corresponding mitochondrial enzyme) were investigated with both propionaldehyde and butyraldehyde as substrates [14].

Analytical, diagnostic and therapeutic context of LOC443343

• Crystallization and preliminary X-ray diffraction studies on cytosolic (class 1) aldehyde dehydrogenase from sheep liver [15].
• Preparations of sheep liver cytoplasmic aldehyde dehydrogenase obtained by published methods were found by analytical isoelectric focusing in the pH range 5--8 to contain 5--10% by weight of the mitochondrial aldehyde dehydrogenase [16].

References