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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs).

5-, 8-, 9-, 11-, 12- AND 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (mu Porasil) column or on a reversed phase (mu Bondapak C18) column. The yield of 5-HPETE was considerably greater when the mu Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20 degrees C, the HPETES were stable for several months.[1]

References

  1. Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs). Boeynaems, J.M., Oates, J.A., Hubbard, W.C. Prostaglandins (1980) [Pubmed]
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