A new assay for the microsomal metabolism of nitrosamines.
This report describes a new general assay for the microsomal oxidative dealkylation of nitrosamines. After precipitation of the microsomal proteins, aldehydes formed from nitrosamines are quantitated by high-pressure liquid chromatography as their 2,4-dinitrophenylhydrazones. This novel assay method offers some distinct advantages over the commonly used Nash reagent assay. Dimethylnitrosamine, diethylnitrosamine, and methylethylnitrosamine were metabolized by microsomes from noninduced male Fischer rat liver, and the aldehydes produced by these reactions were examined. Two distinct kinetic lines were observed for reactions containing dimethylnitrosamine or diethylnitrosamine. Similarly, two Km's were observed for the production of both formaldehyde and acetaldehyde produced by the metabolism of methylethylnitrosamine.[1]References
- A new assay for the microsomal metabolism of nitrosamines. Farrelly, J.G. Cancer Res. (1980) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
