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Ohta, S. et al.

Characterization of a taxol-resistant human small-cell lung cancer cell line.

Taxol is a novel anticancer agent with activity against a broad range of tumors. It has a unique ability to stabilize polymerized tubulin into microtubule bundles within the cell. We have established a taxol-resistant human small-cell lung cancer cell line (H69/ Txl) by exposing H69 cells to stepwise increases in taxol concentration. The resistance of H69/ Txl cells to taxol was 4.7-fold that of the original H69 cells: the IC50 values for H69 and H69/ Txl were 113.7 +/- 56.54 nM and 538.7 +/- 214.7 nM by the tetrazolium dye assay, respectively. Removal of the drug from the medium resulted in a 38% decrease in the growth rate of H69/ Txl as compared with that in the presence of 30 nM taxol, suggesting that the growth of H69/ Txl was partially dependent on taxol. H69/ Txl showed higher sensitivity to vinca alkaloids such as vindesine, vincristine and vinblastine than the parental H69. There was no significant difference in intracellular [3H]taxol content between H69 and H69/ Txl cells. No MDR-1 mRNA was detected in H69/ Txl by the reverse transcription polymerase chain reaction. There was no significant difference of total and polymerized tubulin content between H69 and H69/ Txl cells. Altered mobility of one of the alpha-tubulin isoforms in H69/ Txl was revealed by using isoelectric focusing and Western blotting with anti-alpha-tubulin antibody. In H69, two alpha-tubulin isoforms were observed, whereas three were evident in H69/ Txl, two of them comigrating with the isoforms of H69 and the other being more acidic. We observed the increased acetylation of alpha-tubulin in H69/ Txl cells as compared with that in H69 cells. The acetylation of alpha-tubulin may be responsible for the taxol resistance and/or taxol-dependent growth of H69/ Txl.[1]

References

Characterization of a taxol-resistant human small-cell lung cancer cell line. Ohta, S., Nishio, K., Kubota, N., Ohmori, T., Funayama, Y., Ohira, T., Nakajima, H., Adachi, M., Saijo, N. Jpn. J. Cancer Res. (1994) [Pubmed]

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