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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.

The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB- xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.[1]

References

  1. Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12. White, B.J., Hochhauser, S.J., Cintron, N.M., Weiss, B. J. Bacteriol. (1976) [Pubmed]
 
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