Demonstration of cell division by septation in a variety of gram-negative rods.
Through use of an initial fixative employing a combination of crotonaldehyde and glutaraldehyde, septa were preserved in thin sections of dividing cells of strains of Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, and Escherichia coli when grown at 30 C in a dilute basal medium. The same procedures, however, revealed only a constrictive division process in Proteus vulgaris and Erwinia sp. This adds to the evidence that septation, although difficult to demonstrate, is the process of cell division in the enteric gram-negative rods and the pseudomonads and that constriction is a fixation artifact in these organisms.[1]References
- Demonstration of cell division by septation in a variety of gram-negative rods. Gilleland, H.E., Murray, R.G. J. Bacteriol. (1975) [Pubmed]
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