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Zhang, H.Q. et al.

Gene amplification and high-level expression of human pro-urokinase cDNA in Chinese hamster ovary cells.

Human Pro-Urokinase (Pro-UK) is expressed in CHO-DHFR- cells at high efficiency by co-transfecting the mouse dhfr gene and Pro-UK cDNA under the control of the SV40 late promoter. After gene co-amplification, the product level could reach 2-3 micrograms/10(6) cells/24 h in the presence of 5 x 10(-6) mol/L MTX, and the levels can be further raised to 3.5-4 micrograms/10(6) cells/24 h by PMA superinduction. The copy number of Pro-UK cDNA in the genomes of host cells is about 200-300 copies/cell. The Western blot analysis shows that the recombinant Pro-UK has similar molecular weight to its natural counterpart, and also the amidolytic activity of the product is determined by S-2444 assay.[1]

References

Gene amplification and high-level expression of human pro-urokinase cDNA in Chinese hamster ovary cells. Zhang, H.Q., Li, F.Z., Yu, W.Y., Li, X.Z., Fang, J.M., Fei, E.G., Huang, C.F. Sci. China, Ser. B, Chem. Life Sci. Earth Sci. (1994) [Pubmed]

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