Disease relevance of Chromogenic substrate S-2444

• The ascites PA activated plasminogen and showed amidolytic activity for S-2444 [1].

High impact information on Chromogenic substrate S-2444

• Similar inhibitory effects of hCG on urokinase-plasminogen activator (uPA) and the activity of trophoblast-conditioned media were shown (measured by degradation of S-2444) [2].
• The active 2-chain uPA generated from scuPA by PSA was measured by hydrolyzation of S-2444, a synthetic substrate for uPA [3].
• No difference was found, however, among recombinant and natural scu-PAs in the kinetics of conversion into two-chain active forms (tcu-PAs) by human plasmin, and in the KM and kcat values of tcu-PA activity on the chromogenic substrate S-2444 and on human plasminogen [4].
• Suppression of matrix metalloproteinase activity by SI-27: detection by a new activity assay with S-2444, a specific chromogenic peptide [5].
• However, the plasminogen activator cleaved S-2288 to a greater extent than S-2444 in contrast to urokinase [6].

Biological context of Chromogenic substrate S-2444

• The plasminogen-dependent hydrolysis of the chromogenic substrate S-2444 in presence of trasylol (Bayer, Leverkusen, Germany) was taken as a measure of plasminogen activator activity in these experiments [7].
• The enzyme demonstrated amidolytic activity to Ac-Ile-Glu-Gly-Arg-pNA (S-2423) and related synthetic substrates (S-2222, S-2422, S-2337, and Boc-Leu-Gly-Arg-pNA) but not to other substrates (S-2160, S-2238, S-2251, S-2444, S-2266, and S-2302), indicating specificity similar to mammalian blood coagulation Factor Xa [8].

Anatomical context of Chromogenic substrate S-2444

• Single-chain urokinase-like plasminogen activators have been determined by an indirect method (after activation to urokinase by plasmin and chromogenic assay with S 2444) in the cytosol of a rat tumor model [9].

Associations of Chromogenic substrate S-2444 with other chemical compounds

• In the absence of plasminogen, the activator was devoid of amidolytic activity towards S-2251, S-2302 and S-2288 but had a small but measurable activity against S-2444 [10].

Gene context of Chromogenic substrate S-2444

• Surprisingly, the isolated PCI inhibited the amidolytic activity of urokinase (u-PA) on Glu-Gly-Arg-pNA (S-2444) in a time-dependent manner [11].

Analytical, diagnostic and therapeutic context of Chromogenic substrate S-2444

• UK activity was measured using a chromogenic substrate S-2444 and the antigen by an ELISA kit [12].
• Spectrometric determination of urokinase in urine after gel filtration, using the chromogenic substrate S-2444 [13].
• Also, there was not much difference of the fibrinolytic parameters in terms of the plasma euglobulin clotlysis time, S-2444 amidolysis, and Ma values on thromboelastography [14].
• The Western blot analysis shows that the recombinant Pro-UK has similar molecular weight to its natural counterpart, and also the amidolytic activity of the product is determined by S-2444 assay [15].

References