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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A putative pyruvate dehydrogenase alpha subunit gene from Trypanosoma cruzi.

A full-length DNA clone encoding a putative pyruvate dehydrogenase alpha subunit ( E1 alpha) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1 alpha obtained from different sources. The highest score is obtained with human E1 alpha: 43,3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1 alpha gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA.[1]

References

  1. A putative pyruvate dehydrogenase alpha subunit gene from Trypanosoma cruzi. Buscaglia, C.A., Pollevick, G.D., Veloso, C., Lorca, M., Frasch, A.C., Sánchez, D.O. Biochim. Biophys. Acta (1996) [Pubmed]
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