Identification of the earliest B lineage stage in mouse bone marrow.
We have identified a very early stage of B lineage cells in the CD45R (B220)+CD24 ( HSA) pre-pro-B fraction of mouse bone marrow delineated by expression of AA4.1, a molecule found on stem cells and early B lineage cells. These cells are B lineage precursors based on their capacity to generate B lineage cells rapidly in stromal-dependent culture and their expression of high levels of germline IgH transcripts in the absence of Rag-1/2. Half of these AA4.1+ precursors express low levels of CD4, characteristic of lymphoid progenitors, but few if any have up-regulated CD19, a molecule expressed very early in the B lineage. Furthermore, expression of genes encoding pre-B and B cell receptor components (mb-1, B29, and lambda 5) and transcription factors necessary for B lineage differentiation (BSAP, E12, E47, and Id) provide further support for designating these cells as the earliest B cell precursors.[1]References
- Identification of the earliest B lineage stage in mouse bone marrow. Li, Y.S., Wasserman, R., Hayakawa, K., Hardy, R.R. Immunity (1996) [Pubmed]
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