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Gene Review

Igll1  -  immunoglobulin lambda-like polypeptide 1

Mus musculus

Synonyms: BB139905, CD179 antigen-like family member B, Ig lambda-5, Igl-5, Igll, ...
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Disease relevance of Igll1


High impact information on Igll1

  • Primarily, these mice have few B220+ IgM+ mature B or B220+ CD43- pre-B cells in the bone marrow, reduced frequencies of V(D)J and V kappa J kappa recombination of the immunoglobulin loci, and lower expression levels of the immunoglobulin, RAG-1, RAG-2, and lambda 5 genes [5].
  • The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice [6].
  • A critical role of lambda 5 protein in B cell development [7].
  • We have generated mice in which the lambda 5 gene is inactivated by targeted gene disruption in embryonic stem cells [7].
  • Our findings define a new immunoglobulin L-chain-related locus, which we call lambda 5, that is selectively transcribed in pre-B lymphocytes [8].

Biological context of Igll1

  • We have now found, 4.6 kb upstream of lambda 5, another gene composed of two exons which is selectively expressed in pre-B cell lines as a 0.85 kb mRNA potentially coding for a protein of 142 amino acids including a 19 amino acid-long signal peptide [9].
  • Transgenic human lambda 5 rescues the murine lambda 5 nullizygous phenotype [10].
  • High levels of expression of the transgenic hu lambda 5 gene were detected in bone marrow pro-B and pre-B cells at the mRNA and protein levels, suggesting that the 28-kb transgene fragment contains all the transcriptional elements necessary for the stage-specific B progenitor expression of hu lambda 5 [10].
  • A gene encoding the lambda 5 light chain constant region was isolated from a genomic library from the SPE mouse strain (C lambda 5S) [11].
  • The data indicate that the elements responsible for the enhancer activity (-210 to -22) are part of the lambda 5 gene promoter and likely confer the tissue and stage specificity via positive elements within the -210 to -22 region [12].

Anatomical context of Igll1

  • The murine gene lambda 5 is selectively expressed in pre-B lymphocytes [9].
  • These mice showed severely impaired B cell development which was similar to that previously found in mice lacking either lambda 5 or VpreB1/VpreB2 [13].
  • The VpreB2 gene is functional, as it directed the production in COS cells of a 16-kDa protein that assembled with lambda 5 and was recognized by a VpreB-specific monoclonal antibody [14].
  • They also down-regulate the expression of VpreB and lambda 5, and lose the capacity to proliferate on stromal cells in the presence of IL-7 [15].
  • The chromosomal location of the murine lambda 5 gene was analyzed by Southern hybridization using restriction enzyme-digested DNA from a panel of 15 mouse X hamster somatic cell hybrids [16].

Associations of Igll1 with chemical compounds

  • Interaction of murine precursor B cell receptor with stroma cells is controlled by the unique tail of lambda 5 and stroma cell-associated heparan sulfate [17].
  • Lipopolysaccharide (LPS) stimulation of 70Z/3 pre B cells does not turn off lambda 5 expression [2].
  • Culturing NFS-5.3 with heparin similarly decreases the membrane-form mu; however, it increases the surface level of a pentameric mu molecule containing secreted-form mu (microS) heavy chains, disulfide-linked omega (lambda 5) chains, and noncovalently associated proteins [18].
  • In the presence of a heterologous enhancer, 5' lambda 5 confers pre-B cell-specific expression on the reporter gene chloramphenicol acetyl-transferase [19].

Physical interactions of Igll1

  • A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines [20].

Regulatory relationships of Igll1

  • The VpreB1 protein is thought to be expressed on the surface of pre-B cells in association with lambda 5 and mu heavy chain, and to play an important role on B cell differentiation [21].
  • The lambda 5 protein is expressed in pre-B cells in association with VpreB and mu-heavy chains, and is critical for differentiation to the B cell stage [12].
  • Bone marrow cells from immunoglobulin HC transgenic RAG-2-/- mice have up-regulated expression of germ-line kappa LC gene transcripts and down-regulated expression of lambda 5 surrogate LCs (SLCs) [22].
  • The c-myc protein represses the lambda 5 and TdT initiators [23].

Other interactions of Igll1


Analytical, diagnostic and therapeutic context of Igll1

  • The conservation of the C lambda 5 gene in various Mus species was observed by high stringency Southern blot analyses using a C lambda 5S probe on DNA sample from members of four different groups of wild mice [11].
  • Allelic exclusion at the IgH locus was examined in B lineage cells of wild-type mice and mice unable to express the surrogate light chain molecule lambda 5 using a single-cell PCR approach [27].
  • Using this system and employing mutant mice in which the membrane exon of the mu chain, the lambda 5 gene, or the JH locus was inactivated by gene targeting, we found that expression of the pre-B cell receptor complex is necessary for the transition from the large CD43+ to the small CD43- pre-B cell stage [28].


  1. Organization of the murine Ig-related lambda 5 gene transcribed selectively in pre-B lymphocytes. Kudo, A., Sakaguchi, N., Melchers, F. EMBO J. (1987) [Pubmed]
  2. The expression of the mouse VpreB/lambda 5 locus in transformed cell lines and tumors of the B lineage differentiation pathway. Kudo, A., Thalmann, P., Sakaguchi, N., Davidson, W.F., Pierce, J.H., Kearney, J.F., Reth, M., Rolink, A., Melchers, F. Int. Immunol. (1992) [Pubmed]
  3. B-cell development: a comparison between mouse and man. Ghia, P., ten Boekel, E., Rolink, A.G., Melchers, F. Immunol. Today (1998) [Pubmed]
  4. Pre-B-cell development in the absence of lambda 5 in transgenic mice expressing a heavy-chain disease protein. Corcos, D., Dunda, O., Butor, C., Cesbron, J.Y., Lorès, P., Bucchini, D., Jami, J. Curr. Biol. (1995) [Pubmed]
  5. Constitutive expression of the Id1 gene impairs mouse B cell development. Sun, X.H. Cell (1994) [Pubmed]
  6. The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice. Karasuyama, H., Rolink, A., Shinkai, Y., Young, F., Alt, F.W., Melchers, F. Cell (1994) [Pubmed]
  7. A critical role of lambda 5 protein in B cell development. Kitamura, D., Kudo, A., Schaal, S., Müller, W., Melchers, F., Rajewsky, K. Cell (1992) [Pubmed]
  8. Lambda 5, a new light-chain-related locus selectively expressed in pre-B lymphocytes. Sakaguchi, N., Melchers, F. Nature (1986) [Pubmed]
  9. A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes. Kudo, A., Melchers, F. EMBO J. (1987) [Pubmed]
  10. Transgenic human lambda 5 rescues the murine lambda 5 nullizygous phenotype. Donohoe, M.E., Beck-Engeser, G.B., Lonberg, N., Karasuyama, H., Riley, R.L., Jäck, H.M., Blomberg, B.B. J. Immunol. (2000) [Pubmed]
  11. Conservation of the immunoglobulin C lambda 5 gene in the Mus gene. Mami, F., Cazenave, P.A., Kindt, T.J. EMBO J. (1988) [Pubmed]
  12. Identification and localization of a developmental stage-specific promoter activity from the murine lambda 5 gene. Yang, J., Glozak, M.A., Blomberg, B.B. J. Immunol. (1995) [Pubmed]
  13. VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus. Shimizu, T., Mundt, C., Licence, S., Melchers, F., Mårtensson, I.L. J. Immunol. (2002) [Pubmed]
  14. The murine VpreB1 and VpreB2 genes both encode a protein of the surrogate light chain and are co-expressed during B cell development. Dul, J.L., Argon, Y., Winkler, T., ten Boekel, E., Melchers, F., Mårtensson, I.L. Eur. J. Immunol. (1996) [Pubmed]
  15. Interferon-gamma arrests proliferation and causes apoptosis in stromal cell/interleukin-7-dependent normal murine pre-B cell lines and clones in vitro, but does not induce differentiation to surface immunoglobulin-positive B cells. Grawunder, U., Melchers, F., Rolink, A. Eur. J. Immunol. (1993) [Pubmed]
  16. Localization of the murine lambda 5 gene on chromosome 16. Kudo, A., Pravtcheva, D., Sakaguchi, N., Ruddle, F.H., Melchers, F. Genomics (1987) [Pubmed]
  17. Interaction of murine precursor B cell receptor with stroma cells is controlled by the unique tail of lambda 5 and stroma cell-associated heparan sulfate. Bradl, H., Wittmann, J., Milius, D., Vettermann, C., Jäck, H.M. J. Immunol. (2003) [Pubmed]
  18. Heparin alters the expression of different forms of immunoglobulin mu heavy chains and their associated proteins by pre-B cell lines and normal Ly-1 (CD5+) B cells. Chen, J., Herzenberg, L.A., Herzenberg, L.A. Int. Immunol. (1991) [Pubmed]
  19. Pre-B cell-specific lambda 5 gene expression due to suppression in non pre-B cells. Mårtensson, I.L., Melchers, F. Int. Immunol. (1994) [Pubmed]
  20. A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines. Karasuyama, H., Rolink, A., Melchers, F. J. Exp. Med. (1993) [Pubmed]
  21. A pre-B- and B cell-specific DNA-binding protein, EBB-1, which binds to the promoter of the VpreB1 gene. Okabe, T., Watanabe, T., Kudo, A. Eur. J. Immunol. (1992) [Pubmed]
  22. Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation. Young, F., Ardman, B., Shinkai, Y., Lansford, R., Blackwell, T.K., Mendelsohn, M., Rolink, A., Melchers, F., Alt, F.W. Genes Dev. (1994) [Pubmed]
  23. The c-myc protein represses the lambda 5 and TdT initiators. Mai, S., Mårtensson, I.L. Nucleic Acids Res. (1995) [Pubmed]
  24. SL/KH strain of mice: a model of spontaneous pre-B-lymphomas. Shimada, M.O., Yamada, Y., Nakakuki, Y., Okamoto, K., Fukumoto, M., Honjo, T., Hiai, H. Leuk. Res. (1993) [Pubmed]
  25. Antigenic phenotype and gene expression pattern of lymphohemopoietic progenitors during early mouse ontogeny. Marcos, M.A., Morales-Alcelay, S., Godin, I.E., Dieterlen-Lievre, F., Copin, S.G., Gaspar, M.L. J. Immunol. (1997) [Pubmed]
  26. Lambda5 is required for rearrangement of the Ig kappa light chain gene in pro-B cell lines. Miyazaki, T., Kato, I., Takeshita, S., Karasuyama, H., Kudo, A. Int. Immunol. (1999) [Pubmed]
  27. Surrogate light chain expression is required to establish immunoglobulin heavy chain allelic exclusion during early B cell development. Löffert, D., Ehlich, A., Müller, W., Rajewsky, K. Immunity (1996) [Pubmed]
  28. Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development. Ehlich, A., Schaal, S., Gu, H., Kitamura, D., Müller, W., Rajewsky, K. Cell (1993) [Pubmed]
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