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Xie, Q. et al.

Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.

A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR1-410, the SMR1B gene was confirmed as a convenient dominant selective marker for yeast transformation including industrial strains.[1]

References

Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae. Xie, Q., Jiménez, A. FEMS Microbiol. Lett. (1996) [Pubmed]

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