Disease relevance of BRN 0937935

• Growth of S. typhimurium and E. coli mutants lacking ALS I was prevented by the herbicide, suggesting that activity of the remaining ALS isoenzyme (II or III, respectively) was stopped by sulfometuron methyl [1].
• The insertion and point mutants shared the following phenotypes: resistance to fluoroacetate, sensitivity to alizarin yellow, inability to utilize inositol as a sole carbon source, and hypersensitivity to sulfometuron methyl [2].

High impact information on BRN 0937935

• They have acetolactate synthase activity that is insensitive to sulfometuron methyl because of mutations in or near ilvG, the structural gene for acetolactate synthase isozyme II [3].
• ALS has recently been shown to be the target in bacteria, yeast and plants, of the potent new herbicide sulfometuron methyl [4].
• Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl [5].
• Expression of AtGCN2 in yeast gcn2 mutants complemented the mutation, enabling growth in the presence of sulfometuron methyl, an inhibitor of branched-chain amino acid biosynthesis, and 3-aminotriazole, an inhibitor of histidine biosynthesis [6].
• First, the yeast strain was transformed with plasmid pCAT2 (delta car1 SMR1), and strains heterozygous for CAR1 gene were isolated on sulfometuron methyl plates [7].

Biological context of BRN 0937935

• A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase [8].

Associations of BRN 0937935 with other chemical compounds

• When L-valine, which allosterically inhibits ALS I activity, was added, growth retardation of the relA- strain by sulfometuron methyl was observed on both poor and rich carbon sources [1].
• The electrophoretic behavior of seven sulfonylureas (bensulfuron methyl, sulfometuron methyl, nicosulfuron [accent], chlorimuron ethyl, thifensulfuron methyl [harmony], metsulfuron methyl, and chlorsulfuron) was studied under capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) conditions [9].

Gene context of BRN 0937935

• Chimaeric genes were constructed containing the 5' upstream and partial coding sequence of SMR1--a sulfometuron methyl resistant allele of the ILV2 locus [10].
• During preconcentration by CFLME, five target compounds, including metsulfuron methyl, bensulfuron methyl, tribenuron methyl, sulfometuron methyl, and ethametsulfuron, were enriched in 960 microl of 0.5 mol l(-1) Na2CO3-NaHCO3 (pH 10.8) buffer used as acceptor [11].
• Sulfometuron was detected in streamflow at low levels up to 29 days after treatment (DAT) on the watershed treated with the 75% dispersible granule formulation (Oust; DuPont Chemical Company, Wilmington, DE) and less than 53 DAT on the watershed treated with the experimental formulation (1% pellet) [12].

Analytical, diagnostic and therapeutic context of BRN 0937935

• Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae [13].

References