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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Effect of medium composition on hybridoma growth and antibody production.

Two murine hybridoma cell lines named 1D12 and 3B1 secreting antibody against human blood group B were developed. Effect of medium composition and hyperosmotic stress on cell growth, viability and monoclonal antibody (MAb) production was examined during down-stream process in suspension culture. Both of the cell lines secreted MAb of higher titre if the basal medium was supplemented with 2.5 g/l L-glucose and 419 mg/l L-glutamine. Additional nutrients like transferrine, amino acids, ethanolamine, selenium were added to promote cell growth and viability. In case of cell line 1D12 enhancement of MAb production was observed using hyper-osmotic medium as well. Having data of these preliminary experiments we optimized the large scale production of MAbs in bioreactor. These MAbs serve as a base of the anti-B blood group reagent registered in Hungary.[1]

References

  1. Effect of medium composition on hybridoma growth and antibody production. Hencsey, Z., Fizil, A., Inzelt-Kovács, M., Veszely, G., Bánkúti, L. Acta microbiologica et immunologica Hungarica. (1996) [Pubmed]
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