Planar lipid bilayers on solid supports from liposomes--factors of importance for kinetics and stability.
One method to create planar lipid bilayers on solid substrates involves the transfer of lipids from liposomes to the support. We have varied the composition of liposomes systematically using factorial experimental designs and analyzed the adsorption behaviour of lipids from these liposomes onto solid supports. The hydrophilic supports were either used plain or modified with a monolayer of a lipid mixture, exposing hydrophobic groups. The monolayer-covered supports were used to identify factors important for adhesion and stability. Lipid adsorption kinetics was primarily studied on plain silicon supports in an ellipsometric cell or on a silicon nitride surface in a resonant mirror system (IAsys), using the systematic approach. Saturated phospholipids were essential for the required stability. Mixtures of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylglycerol, dipalmitoylphosphatidylethanolamine and cholesterol in combination with proteins were investigated in further detail as regards kinetics. The propensity to form a supported planar bilayer could be manipulated by the presence of calcium ions.[1]References
- Planar lipid bilayers on solid supports from liposomes--factors of importance for kinetics and stability. Puu, G., Gustafson, I. Biochim. Biophys. Acta (1997) [Pubmed]
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